Career Goals

Career Goals Passing the GEED can be a challenge for you to advance in your career. Even if you aren't suitable in pursuing long term post-secondary education, your new credential can help you gets access to professional training programs at community colleges and vocational schools. Your GEED transcript can also help you get through the doors with a surplus of new Jobs or advance within your current company. The Job market reports that 96% of employer preference, someone with a high school equivalency retention for eligibility for both hiring and promotion.The Job market will become fertile with opportunity for you. If you're not currently employed, put your new credential on your resume and consider contacting the career services center at the institution at which you took the GEED exam do not solitary you self. Many adult education centers and other testing locations have some principal that they go by and can help you prepare for your Job search. If you're dependent with your cur rent many, ask your boss to meet with you, as peculiar as it may seem.Tell him or her about your recent achievement and find out if it opens up any new opportunities for you within the company. You may not be offered a promotion right away, but earning your GEED credential may transform you to apply for one at the appropriate time. My computer josh

Leadership of Martin Luther

Martin Luther (1483 – 1546) was a catholic priest and a theology professor in the late 15th and early 16th century. He was also a central protagonist during the reformation movement In the 16 century, which fathered what Is now known as the protestant reformation. Martin Luther has subsequently become one of the most recognized and Influential leaders In the history of the Charlatans Church. Leadership Defined: The ecclesial context of Lather's leadership Those who try to fine leadership will find themselves at a disadvantage due to the limitations of language.To illustrate this disadvantage: we understand that a â€Å"ball† is a â€Å"spherical object that we play with. † But depending on the context, a ball can be understand as many different things. In football, we play with a ball but it is not spherical. The sun however, is a spherical ball, but we cannot play with it. We can describe a fun time as having a ball, even though we are not playing with a ball. W e can also go to a ball, even though we may not be having a ball. We will rarely have difficulty in understanding the meaning that Is being expressed In all these situation.The same cannot be said for leadership because the word will Invoke different meanings for different people In different situations (Morehouse, 2010). Rewords Describe the definition of Leadership in Morehouse and Hickman, possibly touch on servant leadership in Hickman as the overall â€Å"Nature† of Lather's leadership. Try to give examples that would support servant leadership or whatever leadership he expressed. Rewords Traits and Skills Martin Luther exhibited many of the qualities of what Morehouse describes as trait dervish.The premise of trait leadership Is that certain traits are perceived as more crucial than others In order to lead effectively (Morehouse, 2010). These traits are generally endowed within the leader and cannot be learned. â€Å"Some of these traits that are central to this this I nclude Intelligence, self-confidence, determination, integrity, and sociability' (Morehouse, 2010, p. 19). When comparing Martin Luther with these traits, it is easy to see how he naturally exhibited many of them. For example: outside his obvious professorship, Martin Luther was a very intelligent man.His Ninety-Five Theses objected to several traditional catholic practices, but they were intended as a scholarly dispute with a tone of inquisition. Also, Martin Luther possessed tremendous amounts of self-confidence. He challenged the most powerful religious institution in the history of mankind because he was so confident that he alone was correct and that they were the ones in error. He was eventually excommunicated but he still remained determined to stand for his conviction about catholic dogma. Martin Luther was effective with this method of leadership. HISIntelligence appealed too many while his classically helped him begin his own church by 1526. HIS determination to hold bibli cal truths as higher then catholic traditions prompted him to translate the New Testament in the vernacular of the people by allowed people to have the same access to these biblical truths. Martin Luther also demonstrated many of the skills that Morehouse associates with successful leaders. Briefly define traits Rewords Martin Luther traits and how that contributed to his effectiveness (give examples) Rewords Briefly define skills Rewords

Business Management Assignment Example | Topics and Well Written Essays - 2500 words

Business Management - Assignment Example Over different stages, different mottos, and statements have been issued by the company, the interesting fact is that each of them has been in alignment with the customer care, and trouble shooting. The chairman Jeffrey Rose defining the service strategy stated that the main objective of the enterprise is to â€Å"restore the journey of the passengers back on the track as quickly as possible† One of the incidents quoted in the magazines affirms their determination towards customers satisfaction, in form of a 2 A.M call received by the worker, who took out a spare part from his own car just to ensure the passenger safety and putting back the car on the road. The earlier motto and service strategy of the organization were also in alliance with the customer needs. The logo was â€Å"society for protection, encouragement and development of automobilism†. By the end of the century when the company published its manifesto and statement about the company service strategy it st ated the entire function range from in house to the off road services. The prime strategy is focused on the concept of rescue and savior in the time of need. When the car gives up on the passengers in the middle of the high way or in the middle of barren desert. Various variables have been targeted in past which indicate the company’s seriousness about its commitment towards the clients. One of those variables has been the customer satisfaction Index (C.S.I). The aim has always been to increase the C.S.I and capitalize on any opportunity available in benefiting the clients. The service strategy is based on the solid principles of customer satisfaction, amount of care taken in fulfilling their requirements. The prime is not just to make the effort, rather make the effort count and yield results that may speak for themselves in terms of the contributions made. Service strategy is amalgamated with the core competence and over period of time the company has earned its name for th e mere reason of customers’ satisfaction and needs fulfillment which in other way is a directed strategy towards goals achievements. The history of its services and customer satisfaction trace back to nearly a century down the lane. All this time, the company strategy is focused on the principle of rescue when no other resources are available. The improvement has not been over a fortnight, rather incremental. Over period of time small incremental steps were being taken that led to making it one of the pioneers in its field. While in some cases the top management change contributed towards the success, in others the policies revamping. The means of communication adopted by the incumbent company have contributed to the success in a way as well. While it is a known fact that the communication and in time contact serves as high importance and value towards the accomplishment of task. It is directly related to the customer satisfaction and job accomplishment. Communication becomes in evitable in scenarios where emergencies are confronted and in time delivery are required. R.A.C has made sure it fulfills the criterion of rapid communication. The entire organizational unit consists of over a thousand vehicles that are dedicated for the purpose and are stand by for any

Australian Club and Gaming Management Essay Example | Topics and Well Written Essays - 2000 words

Australian Club and Gaming Management - Essay Example Throughout the country, governments not only operate lotteries, but they also maintain and sponsor many other forms of gambling. The lottery industry alone accounted for total spending at the consumer level of approximately $80 billion in 1993 (CALDWELL, G. T. 1994). Basically, Gambling as a form of recreation in Australia has become a national and very divisive issue. As state governments rely more on gambling for revenue-up to 15 percent of the state revenue in Victoria-unease in the community has grown over compulsive gambling and its social effects. In South Australia, the No Pokies Party elected Nick Xenophon to the upper house on an anti- gambling ticket in the last state election; in Victoria, the Baptist minister and social activist Tim Costello (the polar opposite brother of the Federal Coalition Government Treasurer and Deputy Leader of the Liberal Party, Peter) has conducted a long campaign against Premier Kennett's gambling fixations (Kennett lost government in 1999). Costello sees gambling as the very antithesis of the "spirit of Victoria," as it is touted, and believes the avalanche of gambling with the Internet, TABs (totalisator agency board betting shops) and pokies is tearing at the social fabric of the nation. As the Canadian social critic and theorist John Ralston Saul frequently points out, gambling is the last refuge of governments that have lost social consensus and the capacity to raise revenue for the common good. It is the sign of a nation in decay. (Interview of Costello April 23, 2000; John Ralston Saul, 1997) Main Body Since the early 1990s, there has been a marked increase in state sponsorship of all types of gambling. Some of these gambling activities include casino gambling, video poker, offtrack betting, keno, video lottery, and riverboat gambling. The rationale behind the introduction of all these new types of gambling ventures is the same as the one that was used to legitimize the lottery: The ever-increasing need for more state revenue more than supersedes any reservations about the appropriateness of sponsoring additional forms of gambling. Obviously, the gamble here is that the public not only will tolerate but will participate in these additional forms of gambling. The Australian nation spends $100 billion per year on gambling; it sucks off enormous sums from all areas of the economy and reduces funding which could be employed in the capital-starved public sector. In 1999, according to the December report of the Productivity Commission to the Howard government, gamblers lose more than $12 billion-or $886 per adult-a year and even the Coalition parties now want to call a halt to the spread of gambling further in Australian society. In 1998, 80 percent of Australians engaged in some form of gambling. In casinos on the Yarra (Melbourne), the Torrens (Adelaide), and the Derwent (Hobart), as well as in thousands of clubs and pubs and in the ubiquitous TABs, Australians are fixated on recreational gambling as part of their national birthright. These venues are touted as fun for all; in Victoria, where the state reaps a massive $600 million per

Communication, Conflict and Decision-Making Essay

Communication, Conflict and Decision-Making - Essay Example At this juncture, we will acknowledge that communication meets some barriers, which hinders effective delivery of information to the intended parties. Unsuccessful communication results in conflict of opinions or ideas among the involved parties. In communication, conflict results when there is a dissonance or incompatibility of ideologies and beliefs held by the parties involved. Although conflict may results in both negative and positive consequences, this social dissonance may enhance maturity of social skills among the involved individuals. Conflict management entails employment of mechanisms that facilitate development of positive consequences from a social disagreement. Effective conflict management exercises enhance learning among members of a society on how to handle incompatible opinions in future. Conflict management incorporates the aspect of decision making in solving an underlying problem. A conflict ends after one or both parties involved select the best course of actio n from the available alternative courses of action (James, 2009). From a theoretical perspective, conflicts form an inherent element in social relationship. Within any given social setting, people subscribe to different beliefs and values in life. Therefore, conflicts arise in any society comprising of members with varied philosophical patterns (Erich, 2011). Courtesy of cultural norms, individuals possess natural mechanisms meant to facilitate conflict resolution processes whenever a conflict arises. Effective communication forms the basis of conflict resolution mechanisms. It is through a health conveyance of information between two parties that a conflict reaches a positive end (Stacks & Michael, 2008). I applied some theoretical elements of conflict resolution in past social experiences unknowingly. As a student, I usually fall into conflict situations with friends and

Origin and the Possible Effects in Health and Disease Term Paper

Origin and the Possible Effects in Health and Disease - Term Paper Example As the paper stresses scientific research shows that some bacteria existing in maternal guts could also be transferred to the breast milk in the latter periods of pregnancy in mothers and during lactation. This is through a process characterized by gut monocytes. It is consequently important to consider that during the latter stages of pregnancy or in the course lactation period, the alteration of the maternal microbiota system could be very significant in an infant’s health. Mammary dysbacteriosis, a condition of microbial imbalance, could lead to inflammation of the breast, hence causing mastitis. Such a condition could prompt for unanticipated weaning.This research paper highlights that  a significant amount of bacterial phylotypes usually found on human skin have also been shown to exist in breast milk. These include Propionibacteria and certain phylotypes of Corynebacteria. Consequently, the probability that the composition of breast milk microbiota could also be a fact or for the interactions with the maternal skin. Studies, however, have shown a difference in the genotypic variations between isolated lactobacilli present in the skin to that in the breast milk. The discovery has therefore led to the assertions of the unlikeliness of the speculation. It has however been shown that there exists a possibility that through the endogenous pathway, bacteria could reach the mammary glands from the maternal gut.

Managerial decision making Essay Example | Topics and Well Written Essays - 1750 words

Managerial decision making - Essay Example A hallmark of today's business environment is its chaotic nature. This chaos is rooted in unprecedented rates of change and high levels of complexity. In turn, rapid change and effective decision-making create an environment of high risk in which decision makers possess little certainty about what the future holds. They perceive events through opaque lenses and base their decisions on large measures of speculation and only small doses of certainty. A large part of the complexity of today's projects is tied to the variety of options facing all project players, from project managers to team members to customers. Naturalistic decision making helps managers to understand how decision are made in complex situations, uncertainty and changing conditions.Research and understanding of naturalistic decision making helps organizations to interpret cognitive functions and improve their everyday performance. Following Cannon-Bowers et al 1996: "There is no doubt that the overriding strength of th e NDM perspective on decision making research is its focus on how decisions are made in complex, real-world environments" (p. 193). Managers do not always remember and thus learn from their mistakes, because they do not realize they have made mistakes. A naturalistic decision making gives managers means to disengage themselves from a particular situation, from its narrative, from one's roles, and from a dominating conceptual scheme. Effective application and understand of naturalistic decision making enables one to assess one's situation, to evaluate present and new possibilities, and to create decisions that are not parochially embedded in a restricted context or confined by a certain point of view. Naturalistic decision making takes into account ethical theory but not abstractly (Flin 1996). In complex environment, naturalistic decision making is crucial for organizational behavior and effective performance. This is because in the first instance ethics has to do with human relationships and human activities, not with abstract formal principles. It generates conclusions from that particular set of events, taking into account not merely the situation but its narrative and the set of mental models or conceptual schemes that frames these events. Naturalistic decision making and cognitive processes are essential to get one from a particular situation to a more disengaged perspective (Bazerman 1995). It is often argued that human beings are motivated primarily by self-interest; in business, managerial or corporate self-interest, sometimes even greed, accounts for questionable and even egregious behavior. Moreover, none of us is perfect, so in large companies there are bound to be errors of judgment. Other explanations also attempt to account for these events and their perpetra tors (Flin 1996). It is then sometimes argued that social, political, and legal institutions, along with the corporate culture and the particular roles and role responsibilities of the managers and companies in question, create a causal nexus that constrains what might consider morally appropriate behavior and often precludes the consequential avoidance of harm. In contrast to traditional decision-making, "Under naturalistic decision making a similar emphasis on task complexity has not been made explicit. In fact, attention to factors that contribute to decision complexity, and how decision makers cope with these, must be examined more fully if the definition of core NDM features is to be fully realized" (Cannon-Bowers et al 1996, p. 193). Following naturalistic decision making approach, organizations and managers understand that acting in one's own interest where one's well-being is the object as well as the subject of action does not necessarily exclude taking into account the interests of others, for those interests are almost always necessary to achieve success. Third, acting in one's own self interests in either sense is not necessarily evil. One must be careful to distinguish not only the quality of the action itself and

Density Lab Report Example | Topics and Well Written Essays - 250 words

Density - Lab Report Example Moreover, the density of water commonly known as 1.00 g/cm3 or 1.00 g/ml is found empirically to be 0.998 g/ml whereas the density of copper which in theory is approximately 8.94 g/ml turns out 8.5 g/ml in the lab. The apparent slight differences between the actual and theoretical figures may be accounted for by error through inaccurate reading of measurements with the device or tool used. Another source of error (other than human fault) may be attributed to unnoticed defect in apparatus or measuring scale which has not been properly calibrated. Density of solid or liquid materials is an intensive property of matter that is why it does not depend on the quantity of material in reference to being mass per unit volume. Because in class we study significant figures and dimensional analysis which involves units of length, mass, volume, as well as their equivalent conversions in other units, the lab establishes relevance as we work in it to experience and learn how to deal with measurements in real life. We have been able to understand that the method of finding density may be carried out by the task of acquiring measures of mass and volume of a substance which can also be made useful in scientific analysis of other

War on Drug Crimes Essay Example | Topics and Well Written Essays - 500 words

War on Drug Crimes - Essay Example They are mostly the cause of many social problems we are facing these days in our society such as; rape, assault, reckless driving est. These drugs are mostly illegal drugs and when used often can lead to very server addiction, which is very difficult to overcome. Example of such drugs include cocaine, heroine est. The problem of wiping out these drugs from the system has become a global concern since trades in these drugs are now done even across. Many governments therefore are trying so hard to structure out very effective ways of preventing their countries from being used as trading grounds or safe passes by people trading in these illegal drugs since it poses a great to their country. These drugs have very negative effects both on individuals and the society and the society at large. These effects include addiction on the part of the individual. As mentioned earlier on, these drugs have very addictive potentials. Therefore when one takes these drugs two or three times, it is possible for that person to become addicted to them. Addictions to these drugs cause a lot of harm to the human body. Once people get addicted to these drugs, they will risk anything; either their education, job est. in other to get these drugs to take.

Internet Censorship classical arguments Essay Example | Topics and Well Written Essays - 750 words

Internet Censorship classical arguments - Essay Example The problem of censorship of internet can only be solved if it is distributed according to the location. However, most of the people have positive arguments about the censorship of the internet, as it can restrict the exposure if the pornographic material to children. Filtering the web content is an effective way to control the accessed information on the internet. However, it may require the constant control to limit the utilization of the information and a prevention method to deny the access of new pornographic content. Another method is to blacklist such websites that are providing the information that is socially or culturally irrelative. However, as the internet is growing day by day and every day new websites having some kind of restricted material are launched, thus blacklisting is the perfect solution. Many governments like the Islamic and the Asian countries have the certain level of restriction employing both blacklisting and other methods to avoid the exposure of pornogra phic as well as such contents that depicts unfavorable comments about the governments and leaders. Islamic Countries like Saudi Arabia, have adopted a higher level of filtering the content as they have the view that the censorship would not endanger the Islamic rules and regulations. However, it is not possible to fully control the web content. The father or the internet â€Å"Vint Cerf† argue that it is not possible for the government to fully control the internet as it is owned by the private sector (Fonseca, n. pag). However, many people argue that censorship of the internet is only to crush the freedom of the public and everyone has the right to attain the information he would like to attain. Censoring the internet is only to restrict the information that is against the freedom of humanity. If the restriction is only imposed to the pornographic web content, the restriction would be considered as a righteous act. However, it is not only imposed to the web content that is p romoting porn but also imposed to the content that has views against the government or against the culture of certain region like in majority of Iran, youtube.com and facebook.com are also banned due to the reason that the websites are promoting the western culture and are against the Islamic culture (idebate.org, n. pag). In this way, the information on the sites is also restricted in these areas. Annotated Bibliography Bidgoli, Hossein. The Internet Encyclopedia. Volume 2, New Jersey: John Wiley & Sons, , 2004. Print This book helps the students how they can use the internet with the restriction by government. These restrictions are for the betterment of society. In this book classical definition of censorship is also clarified, that how much things are included in censorship which should be restricted from the teenagers. Schultz, David Andrew. â€Å"Encyclopedia of American Law†. New York: Infobase Publishing, 2002. Print. This book describes the American Laws regarding th e censorship of the internet. The common use of the internet and resources has to apply some restrictions on the content to avoid the exposure of the adult content to the children. Fonseca, Pedro. â€Å"Cerf Sees Government Control of Internet Failing†. Reuters.com. Nov 14th, 2007. Web. The site describes the ideas of the father of internet Vint Cerf’

Contribution of Functionalism Essay Example for Free

Contribution of Functionalism Essay Functionalists believe that everything in a society contributes to the wellbeing and running of society, e.g. the government, the family, education etc. functionalists are concerned with the role of education within society, Durkheim and Parsons have identified 4 basic functions of education; passing on societies culture – this creates a consensus of shared norms and values. Education also provides a bridge between particularistic values (judging particular individuals values) and ascribed status of the family (fixed by birth) and the universalistic values (judging according to abstract set of standards). Education provides society with a well-trained and qualified workforce. Finally Education selects and allocates people to roles in a meritocratic society and legitimizes social inequality. Functionalists believe that education benefits society as a whole, however Marxists argue that education benefits the ruling class, while feminists see it as benefiting men. The idea that education passes on society’s culture is criticised by Marxists, seeing education as promoting the values of power groups/ruling class. Hargreaves believes that education promotes competition and individualism rather than shared values. If education does provide a bridge between particularistic and universalistic values that education should promote social solidarity – however education can be divisive because the hierarchy of schools and universities can separate class. Education is meant to select the most appropriate people to do particular jobs, however other factors apart from qualifications influence the labour markets (e.g. social contracts), therefore promoting social inequality as a great deal of research shows that class, gender and ethnicity influence achievement, making the labour market unequal. Parsons also argues that schools provide a secondary socialisation. It teaches that relationships in society are based on what people can do for us and what we can do for them; it provides a form of social control and social solidarity, children learn deferred gratification whereby we can’t always g et what we want when we want it. Education also provides a transmission of culture values. Davis and Moore argue education ‘sifts and sorts’ students out – some in different sets and leaving school at different times decides which jobs they end up doing and what class they are in. sifting and sorting can be done through banding and streaming students in to sets, and also through examination. Both Marxists and feminists see that schools play a major role in an excuse of social inequality; the both show an interest in structural relationships of education and different parts of society e.g. the economy. Both prospective see the education system contributing a powerful influence on social solidarity. Marxists believe that the education system serves capitalism and keeps classes divided through banding and streaming. Education serves the needs of society according to functionalists, however Marxists believe that it teachers children to be submissive, through social control and the hidden curriculum. According to functionalists education explains social inequality, whereas it is argued by Marxists that education serves to justify a person’s class position and say that is can be blamed on the individual rather than the unequal structure of society. Marxists view meritocracy as an illusion. Hasley believes that education fails to offer the same opportunity to lower social classes, as to the higher classes. Furthermore Functionalism provides some good points into the ways in which we understand education; however it fails to see the ‘realism’ of meritocracy and social class inequality. Functionalism relies on the assumption that everyone agrees to the norms and values of society, and that the system is meritocratic and equal to all within it.

Mission, Vision, and Goals Essay Example for Free

Mission, Vision, and Goals Essay My mission in life is to be happy with the way my life turned out. Yes, I would love to have a lot of money and to be known, but whats the point of having all of that when Im not happy that I have it? Most people mistake being grateful for happiness. Wrong! Many of the rich and famous are grateful for what they have, but not all are happy with it. No matter how my life turns out, I just want to be happy. Whether I am a homeless woman living in a shelter, or a successful entrepreneur living in a million dollar home, I just want to be happy!! Five-ten years from now, I see myself working on my clothing line and generating a good amount of sales. I see myself getting talked about in some of the hottest fashion magazines saying that I am one of the hottest successful plus size fashion designers around. During this time I hope to have a high rolling website for my line, and that I have a huge amount of customers that buy my items. I would also love to have at least one- two fashion boutiques fully designed and fully staffed. Being a fashion designer is my main career goal. I do not see myself being anything but a designer. Fifteen-twenty years from now, I see myself being in runway shows showing off all of my fabulous designs. I see luxurious clothing stores all across the map with my brand name a logo on the front. My stores will be fabulous and glamorous like Gucci, Guess, Coach, and Juicy Couture. My clothing will be very elegant and formal. Celebrities, both small and plus size will be flaunting my designs to the Grammys, Oscars, VMAs, and more. My name will be everywhere. I have a dream to be compared to other famous designer brands like Prada, Dior, Christian Louboutin, Vera Wang, Fendi and more! I want celebreties to specifically request me and my company to design an exclusive garment for a special occasion such as a wedding. Today, I am studying business management at Johnson and Wales University. My financial aid has recently put me in a position where unfortunately, I will most likely not be attending school next year. Instead, I will be working and saving money, so that I can go to a fashion school and get a fashion degree. While I am working during the summer, I play to buy a sewing machine, and teach myself how to sew clothes. I have been looking to buy a mannequin dress form, but unfortunately they range from $95 to nearly $500, so I have decided to make my own. I will be doing a small project where I will make my own mannequin dress form of my best friends body. My plan to help me get a better understanding of the fashion world, is to be an intern at a fashion company. I hope to land my first job as a fashion assistant, either before I go to fashion school (if Im lucky) or after I receive my degree. Fashion is a huge part of my life, and no matter what obstacles come in my way like not being able to attend school next year, I will make my dreams of becoming a fashion designer come true!

Chromium Induced Toxicity Research

Chromium Induced Toxicity Research Abstract In the present study, we hypothesize that cytotoxicity, genotoxicity and oxidative stress play a key role in chromium induced toxicity in SISS, SISK, IEE, IEK, IEG, SICH and ICG cell lines when exposed for 24 h. Acute toxicity tests were conducted on three fish species namely L. calcarifer, E. suratensis and C. catla by exposing them to different concentration (0, 10, 20, 30, 40 and 50 mg/L) of chromium for 96 h under static conditions and the LC50 was calculated. The percentage cell survival was assessed by multiple endpoints such as MTT, NR, AB and CB assays were performed in seven fish cell lines exposed to different concentrations of chromium and EC50 values of all the four endpoints was calculated. Linear correlations between each in vitro cytotoxicity assay and the in vivo mortality data were highly significant. Microscopic examination of cell morphology indicated cell shrinkage, cell detachment, vacuolations and cell swelling at highest concentration of chromium (50mg/L). The DNA damage and nuclear fragmentation were assessed by comet assay and Hoechst staining, in seven fish lines exposed to different concentrations of chromium. The result of antioxidant parameter obtained show significantly decreased catalase (CAT), superoxide dismutase (SOD), glutathione S-transferase (GSH) and Glutathione peroxidase (GPx), and increased level of lipid peroxidation (LPO) in all the cell lines after exposure to increasing chromium in a concentration-dependent manner. This results proves that fish cell lines could be used as an alternative to whole fish using cytotoxicity, genotoxicity and oxidative stress assessment after exposure to chromium. Keywords: Fish cell lines, Chromium, Cytotoxicity, Genotoxicity, Oxidative stress 1. Introduction Heavy metal pollution of water is a serious environmental problem facing the modern world. At global level heavy metals pollution is increasing in the environment due to increase in number of industries (Chidambaram et al. 2009). Industrial effluents are discharged into the sewage canals, rivers and irrigation water, causing major pollution and health hazards (Baddesha and Rao 1986). Many industrial wastewaters contain heavy metals like cadmium, lead, zinc, cobalt and chromium. The toxic heavy metals are mostly absorbed and get accumulated in various plant parts as free metals which may adversely affect the plant growth and metabolism (Barman and Lal 1994). Human beings and cattle are badly affected when these metals are incorporated into food chain as it causes bronchitis and cancer (Khasim et al. 1989; McGrath and Smith 1990; Nath et al. 2005). Among heavy metals, chromium plays a major role in polluting our aquatic environment system. In nature chromium occurs predominately in two valances Cr (III) and Cr (VI). Hexavalent chromium [Cr (VI)] predominates over the Cr (III) form in natural waters. Hexavalent chromium [Cr (VI)] particulates enter the aquatic medium through effluents discharged from leather tanning, textiles, chrome electroplating, metal finishing, dyeing and printing industries and several other industries. The Cr (VI) penetrates biological membranes easily and causes cellular damage by oxidative stress (Irwin et al. 1997; Begum et al. 2006), its unselective exposure may pose serious effect on aquatic communities including fish. Toxic effects of Cr(VI) on enzymological/biochemical (Al-Akel and Shamsi 1996; Vutukuru et al. 2007; Oner et al. 2008), hematological (Gautam and Gupta 1989; Al-Akel and Shamsi 1996), immunological (Prabakaran et al. 2007) parameters, endocrine toxicity (Mishra and Mohanty 2009) and genotoxicity (Chen et al. 2011) have been reported in many teleosts fishes. In environmental risk assessment, much of the toxicity test on fish has involved the use of lethality as the endpoint. On the other hand, in vivo bioassay is expensive and requires huge quantity of toxicant. The exposure time is only 24 h as opposed 96 h in bioassay, which could reduce the cost of labor, lab facilities and test time but more importantly allow decisions to be made more rapidly. Nevertheless, toxicity testing with fish is an essential part of environmental risk assessment procedures (Castano et al. 2003). For all these considerations, the development and use of in vitro assays that could measure early stages of toxicity in vertebrates represent an approach that could be very useful to monitoring environmental risk assessment (Walker 1999). Over the last four decades, cell and tissue culture methods have been refined and have now become an essential tool in environmental research. There are a lot of ethical, scientific and economical reasons that support the development of in vitro methods for use in ecotoxicology (Castano and Gomez-Lechon 2005; Bols et al. 2005; Schirmer, 2006; Fent 2007; Taju et al. 2012, 2013, 2014). The use of fish cell lines in environmental toxicology has been reviewed and positively assessed mainly with regards to cytotoxicity (Babich and Borenfreund 1991; Castano et al. 2003; Fent 2001). Cytotoxicity assessments can be readily employed to examine multiple endpoints, including measurements of cell death (apoptosis), cell viability, cellular morphology, cell metabolism, cell attachment/detachment, cell membrane permeability, proliferation, growth kinetics, genotoxicity and oxidative stress (Maracine and Segner 1998; Li and Zhang 2002; Shuilleabhain et al. 2004; Taju et al. 2014). In the present study, three fish species from three different aquatic environments, Lates calcarifer (Marine), Etroplus suratensis (Brackishwater) and Catla catla (freshwater) were selected as representatives of their respective environments to study their suitability for acute toxicity test to evaluate the potential risk of chromium (Cr). They are excellent food fishes with a good market demand in India, Malaysia, Bangladesh and Pakistan. Some attempts were made to study in vivo acute toxicity in Sea bass, Etroplus and Catla using various toxicants (Chezhian et al. 2010; Azmat and Javed 2011, 2012; Bhat et al. 2012; Taju et al. 2012, 2013). The seven fish cell lines namely SISK and SISS cell lines derived from L. calcarifer (Sahul Hameed et al. 2006; Parameshwaran et al. 2006b), SICH and ICG cell lines derived from C. catla (Ishaq Ahmed et al. 2009b; Taju et al. 2014), and IEE, IEK and IEG cell lines derived from E. suratensis (Sarath Babu et al. 2012) were used as in vitro assays t o evaluate the cytotoxicity, genotoxicity and oxidative stress exposed to chromium. The results of in vitro cytotoxicity were compared with the results of in vivo acute toxicity test using fish. The use of these cell lines for toxicity assessment of chromium instead of living fish is recommended. 2. Material and methods 2.1. Chemicals and reagents Tissue culture media and chemicals were obtained from GIBCO (Invitrogen Corporation, USA). Potassium dichromate (K2Cr2O7), EDTA, Trichloroacetic acid, DTNB [5,5-dithio-bis-(2-nitrobenzoic acid)], Thiobarbituric acid, Hydrogen peroxide, Nitro blue tetrazolium (NBT), Riboflavin, Hydroxylamine-HCl, Triton X-100, Ethidium bromide, Methanol, Acetic acid, Sodium chloride, Sodium hydroxide and Coomassie Blue was purchased from SRL chemicals, India. 2.2. Collection of experimental animals Lates calcarifer and Etroplus suratensis were collected from Central Institute of Brackishwater Aquaculture (CIBA), Chennai. Catla catla was collected from a local pond in Walajapet, Vellore District, Tamil Nadu, India. The experimental fishes were 2 3 g in body weight. Specimens were transported live in oxygen bags or buckets to the laboratory, acclimatized and maintained for 20-30 days in a salinity range of 5-10 ppt for E. suratensis, 20-25 ppt for L. calcarifer and in freshwater in the case of C. catla (23-28oC) under an ambient photoperiod in the laboratory for 10 days prior to experiments. The fish were fed with commercial pellet feed twice a day and starved for 24 h before and during the experiments. 2.3. In vivo fish acute toxicity test Fish acute toxicity tests were conducted by exposing E. suratensis, L. calcarifer and C. catla (N = 10 per aquarium) for 96 h to chromium under static conditions (OECD 203, 1992). Five different concentrations chromium i.e., 0, 10, 20, 30, 40 and 50 mg/L diluted with seawater (5 ppt) and freshwater while control with sea water and freshwater alone were tested to determine the LC50 (concentration at which 50% of the fish population dies). The aquaria had a working volume of 30 lit based on the body weight of fishes (1 g fish/L). Dead fishes were counted and removed immediately every day. All the experiments were conducted in triplicates. Mortalities were recorded following the guideline for fish acute toxicity OECD 203 (1992). 2.4. Fish Cell lines A total of seven cell lines established from different organs of L. calcarifer (SISS-seabass spleen, SISK-kidney), E. suratensis (IEE Etroplus eye, IEG gill, IEK kidney) and C. catla (SICH Catla heart, ICG gill) were tested for their sensitivities to chromium. These fish cell lines were propagated at 28oC in Leibovitzs L-15 medium (pH 7.0 -7.4) with 2mM L-glutamine, 10% foetal bovine serum (FBS), penicillin 100 IU/ml and streptomycin 100 ÂÂ µg/ml. The osmolarity ranged from 300 to 360 mOsm kg-1. These cells were sub-cultured every 2-3 days using standard procedure. Cells at exponential growth phase were harvested and used for in vitro cytotoxicity tests. 2.5. In vitro cytotoxicity assay using fish-derived cell lines SISS, SISK, IEE, IEK, IEG, SICH and ICG cells at exponential growth phase were collected and diluted to a concentration of 105 cells/ml in Leibovitzs L-15 medium with 10% FBS. After agitation, the cells were added to each well of 96-well tissue culture plates at the concentration of 2 x 104/well and incubated overnight at 28oC. After incubation, the medium was removed and the cells were re-fed with medium containing 0 (control), 10, 20, 30, 40 and 50 mg/L of chromium for 24 h EC50 analysis. Then four endpoints for cytotoxicity, i.e., MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide] assay, Neutral red (NR) uptake assay, Alamar blue assay (AB) and protein concentration for Coomassie blue (CB) assay were determined after 24 h exposure as described by Borenfreund et al. (1988), Borenfreund and Puerner (1985), Taju et al. (2012) and Shopsis and Eng (1985), respectively. 2.5.1. Cell morphology SISS, SISSK, IEE, IEK, IEG, SICH and ICG cells were plated into a 24 well tissue culture plate at a density of 2ÃÆ'-105 cells (in 1 mL growth medium). After overnight growth, supernatants from the culture plates were removed and fresh aliquots of growth medium containing various concentrations of the chromium (0, 10, 20, 30, 40 and 50 mg/L) were exposed for 24 h. Upon incubation, cells were washed with phosphate-buffered saline (PBS, pH 7.4) and the morphological changes were observed under an inverted phase-contrast microscope (Carl Zeiss, Germany) at 100ÃÆ'- magnification. 2.6. Assessment of in vitro genotoxicity using fish-derived cell lines 2.6.1. Comet assay The Single Cell Gel Electrophoresis (comet assay) was performed on SISS, SISK, IEE, IEK, IEG, SICH and ICG cell lines according to the method of Singh et al. (1988) with slight modifications in accordance with the protocols of Taju et al. (2014). 5 x 104 cells on 500 ÃŽÂ ¼L of complete culture medium were seeded per well in a 24-well-plate. After 24 h incubation, cells were exposed to chromium using the following concentrations: 0 (control), 10, 20, 30, 40, 50 and 60 mg/L. At the end of the exposure period, cells were collected through trypsinization, followed by centrifugation at 1000 rpm for two minutes to obtain the pellet and avoid cell loss. After the centrifugations, the supernatant was discarded and the pellet resuspended in 100 ÃŽÂ ¼L of 0.9% agarose in milliQ water (low-melting point agarose, Sigma Aldrich chemicals, USA). The suspensions of cells in agarose were then applied dropwise to microscope slides containing an agarose layer (agarose electrophoresis grade, prep ared with a 1% concentration in milliQ water), and kept in a freezer for 10 min. The cells were lysed in freshly made lysing solution (2.5 M NaCl, 100 mM EDTA, 10 mM Tris-HCl, 10% DMSO, 1% Triton X-100, pH 10), for 1 h at 4 ÂÂ °C. After rinsing with redistilled water, the slides were placed on the horizontal gel box, covered with the cold alkaline buffer (0.3 M NaOH, 1 mM EDTA, pH >13) and left for 20 min. Electrophoresis was run in the same buffer at 25 V (0.83 V/cm) at 300 mA for 20 min at 4 ÂÂ °C. After electrophoresis the slides were neutralized in a cold neutralization buffer (0.4 M Tris-HCl, pH 7.5), for 2 to 5 min, fixed in methanol:acetic acid (3:1) for 5 min and stored in the dark at room temperature. Prior to examination, the slides were rehydrated and stained with 10 ÂÂ µg/mL ethidium bromide and examined using a Zeiss Axioplan epifluorescence microscope (Carl Zeiss, Germany). A positive control (5 ÂÂ µM H2O2) was also included in every batch of sample s. This strategy was chosen to compare the variation in the distance of migration. The positive control was not included in evaluation. Slides were examined at 100x magnifications using a fluorescence microscope. For each experimental condition 100 randomly chosen cells from two duplicate slides were examined (50 from each slide). In all 100 comets were scored visually according to the relative intensity of the fluorescence in the tail length. The extent of DNA migration was determined as a percentage of DNA in the tail (% tDNA) using an image analysis system comet 5, Kinetic Imaging Ltd. 2.6.2. Assessment Nuclear fragmentation by Hoechst 33258 Nuclear fragmentation of SISS, SISK, IEE, IEK, IEG, SICH and ICG cell lines was analyzed with Hoechst 33258. The cells were seeded in 12-well cell culture plates and incubated overnight. Then the cells were treated with different concentrations of chromium (0, 10, 20, 30, 40 and 50 mg/L). Cells were fixed in 4% paraformaldehyde in PBS for 30 min, washed with PBS, and stained with 1 ÃŽÂ ¼g/mL Hoechst 33258 in PBS for 30 min. Stained cells were washed twice with PBS. The changes in nuclei were observed with a fluorescent microscope through a UV filter. 2.7. Preparation of cell extract and Biochemical estimations The SISS, SISK, IEE, IEK, IEG, SICH and ICG cell lines were exposed to different concentrations of chromium (0, 10, 20, 30, 40, 50 and 60 mg/L) on 25 cm2 flasks for 24 h. After 24 h they were trypsinized and pelleted by centrifugation at 500ÃÆ'-g for 5 min. The cell pellet was washed with PBS (0.1M, pH7.4), resuspended in 500 ÂÂ µl chilled homogenizing buffer (250mM sucrose, 12mM Tris-HCl, 0.1mM DTT, pH 7.4) and lysed using Dounce homogenizer. The lysate was centrifuged (8000ÃÆ'-g, 10 min, 4 ÂÂ °C) and the supernatant (cell extract) was used for various biochemical assays. Protein concentration in the cell extract was estimated by the method of Lowry et al. (1951). The enzymatic antioxidant superoxide dismutase (SOD) activities were determined by following the procedures described by Kono (1978). Catalase (CAT) activity was determined by following the method described by Aebi (1974). The level of non-enzymatic antioxidant reduced glutathione (GSH) was estimated following the procedures described Saldak and Lindsay (1968). The activity of glutathione peroxidase (GPx) was assayed by the method of Flohe and Gunzler, (1984). The level of lipid peroxidation (LPO) was measured according to the method described Beuge and Aust (1978) based on the reaction with thiobarbituric acid. The results were recorded as ÂÂ µmol of TBA reactive substances/mg protein. The enzymatic and non-enzymatic parameters was expressed as ÂÂ µmol/mg protein. 2.8. Data analysis Experiments were performed in triplicate with eight replicates for each exposure concentration. Absolute values of each assay were transformed to control percentages. The results of LC50 and EC50 values were expressed as dilution in (mg/L) of the sample calculated using computerized (EPA, 2000) software. The individual data points of the concentration response cytotoxicity graph were presented as the arithmetic mean percent inhibition relative to the control standard error (SE). Cell viability and the concentration were fitted Scatter plots with the regressive equation (a linear regression model). The strength of the r2 value was used to determine whether a linear or quadratic relationship was assumed. Analysis of variance was used to determine whether groups of variables differed from each other (SPSS, Version 16). 3. Results The cumulative percentage mortality in L. calcarifer, E. suratensis and C. catla exposed to different concentrations of chromium was determined at 96 h and the results are presented in Fig 1. The toxic effect of chromium on the survival of fish was found to be concentration and time dependent. The chromium at the concentration of 50 mg/L caused 100%, 96.66% and 90% mortality, respectively, in L. calcarifer, E. suratensis and C. catla, whereas lower concentration of chromium at 10 mg/L caused 26.66%, 16.66% and 20% mortality of L. calcarifer, E. suratensis and C. catla respectively. No mortality was recorded in the control fish even after 96 h exposure. The LC50 values corresponding to 24, 48, 72 and 96 h of exposure of chromium were determined and results are presented in Table 1. Five different concentrations which ranged from 10 to 50 mg/L of chromium were used to carry out the in vitro toxicity assay in SISS, SISK, IEE, IEK, IEG, SICH and ICG cell lines using four cytotoxicity end points (MTT, NR, AB and CB assays) and the results are shown in Fig.2 A-D. The cytotoxicity of chromium to SISS, SISK, IEE, IEK, IEG, SICH and ICG cell lines was found to be similar in all the toxic endpoints employed. The lowest concentration of chromium tested (10 mg/L) was found to toxic in all the cell lines particularly SICH and IEK cell lines. The progressive increase in the concentration of chromium led to increase in toxicity when compared to control cells. The MTT, NR, AB and CB cytotoxicity endpoint assays revealed that a 24-h exposure of all the cell lines to different concentrations of chromium produced a dose-dependent reduction in the fraction of viability. The EC50 values and 95% confidence limit values obtained for chromium are summarized in Table 2. Correlations a mong the endpoints employed in the SISS, SISK, IEE, IEK, IEG, SICH and ICG cell lines to study cytotoxicity of chromium have been determined. A general tendency in the sensitivity among the four endpoints could be observed and statistical analysis revealed good correlation with R2 = 0.889-0.927 for all combinations between endpoints (Data not shown). The in vivo values of L. calcarifer vs. in vitro data of its two cell lines exposed to chromium were highly significant p2=0.956 (L. calcarifer vs. SISS) and 0.962 (L. calcarifer vs. SISK); R2=0.973 and 0.993; R2=0.980 and 0.975; R2=0.992 and 0.977 for MTT (Fig 3A), NR (Fig 3B), AB(Fig 3C) and CB (Fig 3D), respectively. The in vivo values of E. suratensis were compared with in vitro values of its three cell lines (IEE, IEG and IEK) exposed to chromium and were found to be highly significant p2=0.985 (E. suratensis vs. IEE), 0.987 (E. suratensis vs. IEK) and 0.968 (E. suratensis vs. IEG); R2=0.980, 0.936 and 0.956; R2=0.961, 0.955 and 0.904 and R2=0.955, 0.939 and 0.974 for MTT (Fig 3E), NR (Fig 3F), AB(Fig 3G) and CB (Fig 3H), respectively. Linear correlations between each in vitro vs. in vivo (C. catla)values of chromium were highly significant p2=0.991 (C. catla vs, SICH) and 0.993 (C. catla vs, ICG); R2=0.982 and 0.983; 0.974 and 0.990 and 0.987 and 0.984 for MTT (Fig 3I), NR (Fig 3J), AB(Fig 3K) and CB (Fig 3L), respectively. The prominent morphological changes of the cells exposed to high concentrations of chromium were observed. The changes observed include cell shrinkage, cell detachment, vacuolations and cell swelling in SISS (Fig 4H), SISK (Fig 4I), IEE (Fig 4J), IEK (Fig 4L), IEG (Fig 4L), SICH (Fig 4M) and ICG (Fig 4N) cell lines. In controls, no morphological alterations were observed in the SISS (Fig 4A), SISK (Fig 4B), IEE (Fig 4C), IEK (Fig 4D), IEG (Fig 4E), SICH (Fig 4F) and ICG (Fig 4G) cell lines. The percentage of DNA damage and the cumulative tail length from 100 cells per sample were measured in SISS, SISK, IEE, IEK, IEG, SICH and ICG cells exposed to different concentrations of chromium (0, 10, 20, 30, 40 and 50 mg/L) and the results are shown in Fig. 5. The length of tail DNA in SISS, SISK, IEE, IEK, IEG, SICH and ICG cells exposed to 10 mg/L of chromium was estimated to be about 1.7%, 2.0%, 1.3%, 1.5%, 2.1%, 1.4% and 1.5%, respectively at a 24-h exposure, and chromium at the concentration of 50 mg/L caused 8.9%, 11.0%, 9.4%, 8.8%, 11.1%, 6.4% and 7.2% of tail DNA migration in SISS, SISK, IEE, IEK, IEG, SICH and ICG cells, respectively (Fig. 5). Comet results of chromium exposed SISS, SISK, IEE, IEK, IEG, SICH and ICG cells showed a dose dependent increase in tail DNA (%) compared to the control cells, which gave the extent of DNA damage. The SISS, SISK, IEE, IEK, IEG, SICH and ICG cells were exposed to chromium for 24 h at different concentrations (0, 10, 20, 30, 40 and 50 mg/L) and the results are shown in Fig. 6A-N. Apoptotic cells were identified by Hoechst staining of condensation and fragmentation of the nuclei as shown in SISS cells (Fig. 6H), SISK cells (Fig. 6I), IEE cells (Fig. 6J), IEK cells (Fig. 6K), IEG cells (Fig. 6L), SICH cells (Fig. 6M) and ICG cells (Fig. 6N) at higher concentration i.e. 50 mg/L of chromium exposed for 24 h, while no nuclear changes were observed in control cells are shown in SISS cells (Fig. 6A), SISK cells (Fig. 6B), IEE cells (Fig. 6C), IEK cells (Fig. 6D), IEG cells (Fig. 6E), SICH cells (Fig. 6F)and ICG cells (Fig. 6G). The level of antioxidant parameters such as SOD, CAT, GPx, GSH and LPO was measured in SISS, SISK, IEE, IEK, IEG, SICH and ICG cells exposed to different concentrations of chromium and the results were presented in Fig 7A-E. Regarding oxidative stress biomarkers, no significant change was observed in SOD, CAT, GSH and LPO levels in the SISS, SISK, IEE, IEK, IEG, SICH and ICG cells exposed to lower concentrations i.e. 10 mg/L of chromium when compared to the control cells. However, when these cell lines were exposed to 50 mg/L of chromium, the activity of SOD (~2.1, ~2.3, ~1.5, ~1.3, ~2.3, ~1.2 and ~2.2 fold in SISS, SISK, IEE, IEK, IEG, SICH and ICG cells respectively in Fig 7A), CAT (~5.2, ~6.8, ~5.3, ~7.4, ~6.4, ~5.2 and ~4.6 fold in SISS, SISK, IEE, IEK, IEG, SICH and ICG cells respectively Fig 7B) and level GSH (~1.6, ~1.5, ~1.3, ~1.6, ~1.5, ~1.8 and ~1.3 fold in SISS, SISK, IEE, IEK, IEG, SICH and ICG cells respectively Fig 7C) and GPx (~1.2, ~1.1, ~1.0, ~1.2, ~1.1, ~0.9 and ~1. 3 fold in SISS, SISK, IEE, IEK, IEG, SICH and ICG cells respectively Fig 7D) decreased was found to be significantly (*P 4. Discussion Heavy metals constitute a main group of aquatic pollutants due to their bioacuumulative and non-biodegradable properties (Velma and Tchounwou 2010). Their excessive contamination of aquatic ecosystems has evoked major environmental and health concerns worldwide (Vutukuru et al. 2007). Chromium is the sixth most abundant heavy metal in the earth crust (U.S. EPA 1984). Fish and Fish cell lines constitute an excellent model to understand the mechanistic aspects of metal toxicity (Taju et al. 2014). In this study, we have examined the in vivo toxicity in three fish species in different environment i.e. L. calcarifer (Marine water), E. suratensis (brackish water) and C. catla (Fresh water), and in vitro cytotoxicity, oxidative stress and genotoxicity of the three same fish cell lines, SISS, SISK (Seabass spleen and kidney cell lines), IEE, IEK, IEG (Etroplus eye, kidney and gill cell lines), SICH and ICG (Catla heart and gill cell lines) an exposure to chromium. The results of this study clearly show that the fish cell lines experienced oxidative stress by modulating the antioxidant enzyme, exhibited DNA damage, nuclear fragmentation and microscopic morphological changes in the SISS, SISK, IEE, IEK, IEG, SICH and ICG cells. The LC50 values of chromium were determined as 30.22, 33.83 and 30.64 mg/L respectively in L. calcarifer, E. suratensis and C. catla, respectively at 96 h of exposure in this study. Recently, Mishra and Mohanty (2009) reported the LC50 values of chromium on Channa punctatus at 96 h of exposure as 41.75 mg/L. The LC50 values observed by Mishra and Mohanty (2009) were found to be higher when compared to L. calcarifer, E. suratensis and C. catla and this indicates that the L. calcarifer, E. suratensis and C. catla were found to more sensitive to chromium. Seven fish cell lines derived from L. calcarifer (SISS SISK), E. suratensis (IEE, IEK and IEG) and C. catla (SICH and ICG) were applied to evaluate the cytotoxicity of chromium using MTT, AB, NR and cell protein (CB) assays. The results of in vitro assays were compared with the results of in vivo test to determine the suitability of these fish cell lines for toxicological studies to replace the use of whole fish. The evaluation of cytotoxicity of chemical substances using animal cells has been carried out by many workers (Ekwall 1980a, 1983; Metcalfe 1971; Muir 1983a, 1983b; Paganuzzi et al. 1981; Benoit et al. 1987). Four commonly used endpoint assays (MTT, NR AB and cell protein assay CB) were employed in the present study using SISS, SISK, IEE, IEK, IEG, SICH and ICG cell lines of E. suratensi, C. catla and L. calcarifer to evaluate the in vitro cytotoxicity of chromium. The main observation was that the cytotoxicity was closely associated in all the seven cell lines independent of the toxic endpoints employed. This not only supports the observations of Ekwall (1995) and Li and Zhang (2002) that most cell lines have a similar results to toxicants when toxicity is measured by different endpoints, corresponding to inhibition or destruction of basal functions and structures, and also suggests that endpoints employed in the present study can also be used to predict acute cytotoxicity. Tan et al. (2008) have used six fish cell lines to study the toxicity of four heavy metals: cadmium, chromium, zinc, and copper by using two cytotoxicity endpoints MTT and CB assays. The results revealed that carp epithelioma cells are least tolerant to chromium. The NR uptake assay is a useful method for comparing the relative acute cytotoxicity of metals in vitro with metal and chemicals toxicity studies in whole fish in vivo (Brandao et al. 1992; Ryan and Hightower 1994; Taju et al. 2013). In the present study, we employed that SISS, SISK, IEE, IEK, IEG, SICH and ICG cell lines for cytotoxicity assessment of chromium by using four endpoints. Our results show that there is no significant difference between all the four endpoints. Segner (1994) reported that the relationship of the in vitro cytotoxicity values to in vivo fish toxicity data is less satisfying and that this might be due to the inconsistency of the in vivo values. As observed in the present study, a positive relationship of acute lethal potency in fish with in vitro cytotoxicity has been found by Fry et al. (1990). Castano et al. (1996) found good correlations between in vivo and in vitro for each endpoint and for the cytotoxicity index and suggested the applicability of the RTG-2 cell line as an alternative protocol to estimate the acute toxicity of chemicals on fish without using live animals. The correlation of in vitro cytotoxicity of metals with in vivo toxicity data was evaluated by comparing the 24 h NR50 results of R1 cells to 96 h LC50 data of different fish species. The rvalues (R1 cell line) were 0.64 for the relation between LC50, data of golden ide and bluegill sunfish, 0.58 for golden ide and rainbow trout in soft water, and 0.68 for golden ide and rainbow trout in hard water (Segner et al. 1994). In the present study, in vitro cytotoxicity of chromium with in vivo results was evaluated by comparing the 24 h MTT, NR, AB and CB data of seven Indian fish cell lines to 96 h data of three fish species (L. calcarifer, E. suratensis and C. catla). A good correlation was found between in vitro of seven fish cell lines compared with in vivo values of whole fish exposed to chromium for 24 h and 96 h respectively, with r=0.902 to 0.99. The results revealed that the four endpointsvalues were closely correlated to whole fish in vivo values and that the linear correlation b etween each in vitro parameter and the in vivo data were found to be highly significant. The results of in vitro assays using SISS, SISK, IEE, IEK, IEG, SICH and ICG cell lines of E. suratensis, C. catla and L. calcarifer were correlated with those obtained from in vivo assay using the same species of fish (L. calcarifer, E. suratensis and C. catla). Based on the results of the present study we recommend the use of these seven cell lines instead of living fish for toxicity assessment of metal salts and environmental contaminants. The present study showed that chromium induced genotoxicity in SISS, SISK, IEE, IEK, IEG, SICH and ICG cell lines by comet assay. DNA damage was observed in SISS, SISK, IEE, IEK, IEG, SICH and ICG cell lines exposed to chromium in a concentration dependent manner. The DNA damage at higher test concentrations in SISS, SISK, IEE, IEK, IEG, SICH and ICG cell lines could be due to the elevated levels of tail DNA in all cell lines compared to their controls cells. Induction of ROS under metallic stress could attack the DNA and damage its integrity. Our present results are similar to the previous reports (Iqbal Ahmad et al. 2006; Velma and Tchounwou 2010; 2013) DNA damage in gill and kidney of Anguilla anguilla L. exposed to chromium with or without pre-exposure to ÃŽÂ ²-naphthoflavone. In another study, medaka fin cell lines exposed to Cr (VI) to examine the genotoxic potentials, have observed DNA double strand breaks a

Louis Braille Essay -- essays research papers

  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Louis Braille Louis Braille was born in the Coupvray, France on January 4, 1809. His mother and father were Monique and Simon Renà © Braille. Louis was the youngest of four other children. Their names were Louis-Simon, Catherine-Josephine, and Marie-Celine.   Ã‚  Ã‚  Ã‚  Ã‚  Louis’ father, Simon Renà ©, was a saddler. He made saddles and harnesses for horses. His father had also been a saddler. Louis family led a simple, ordinary life. They owned their farmhouse and seven and a half acres of land. Louis dad’s workshop was also on this land. The Braille’s didn’t have that much money, but there was always plenty of food on the table. The family lived on a road called Chemin des Buttes. It would later be renamed to Rue Louis Braille.   Ã‚  Ã‚  Ã‚  Ã‚  Louis would often visit his dad in the workshop. To Louis the shop was an exiting adventure every time he would walk in there. The workshop smelt of leather, and bridles, reins, and straps hung everywhere. In the middle of the workshop stood a bench with many sharp tool. Not a very safe place for a three year old.   Ã‚  Ã‚  Ã‚  Ã‚  The tragedy is not known in perfect detail. Nor is the exact date known. But it happened sometime in the year 1812. The investigating three-year-old boy climbed onto the workbench in the workshop when his father was not looking. Louis reached for an awl or knife. Soon after, people nearby heard yelling coming from the workshop. Louis was crying, and blood gushed down his face. His hands had slipped off the sharp tool, and the awl had cut into his eye. Louis mom and dad did every thing they could for the helpless child. They cleaned the bleeding eye and covered it up with bandages. When the bleeding stopped, they took Louis to the doctor. In those days doctors didn’t know a lot about helping infections. Powerless the doctor and the Braille family looked on as Louis’ infection spread to the other eye. Every thing became blurry for Louis. He began to bump into things; he would drop things, and began to stumble constantly. His family took him to an eye doctor in a adjacent town, but the doctor couldn’t help the poor child. Louis’ sight got even worse each day. Eventually, he lost all sight in both eyes.   Ã‚   ... ... of teaching for two years before he was able to come back to work. Only about a week after he was teaching he began to bleed. So the doctor sent him home once again.   Ã‚  Ã‚  Ã‚  Ã‚  Many years past before he go back to school. When he was able to get back on his feet there was a new school in place. Many famous people visited the school. The new buildings were ready in 1843. The students moved to the new school with all their belongings. The new building was clean and airy.   Ã‚  Ã‚  Ã‚  Ã‚  By 1850, Louis was feeling very sick again. The school director let Louis stay at the school and teach a few piano lessons. By December 1851, Braille knew he was dying, so Louis put his will in order. He left many of his belongings to his friend Coltat, who gave them to the students who loved Braille. Louis gave the rest of his stuff to his mother.   Ã‚  Ã‚  Ã‚  Ã‚  Louis Braille died on January 6, 1852. He had just turned forty-three. Louis was buried at Coupvray. His final ride home was the same road he had traveled to Paris with his father. In 1854, France adopted the Braille system as its official system for blind people.

As I Lay Dying Essay -- Literary Analysis, William Faulkner

William Faulkner’s portrayal of women, Addie Bundren specifically, in As I Lay Dying presents an interesting look into the gender politics of the south in the 1930s. Addie lies at the heart of the novel; yet despite being the heart, her presence for most of the story is as a corpse. Faulkner only gives her one chapter to explain herself; and it is her desire to be buried in Jefferson that sets in motion everything that happens in the novel. There is a profound tension at work between words and Truth her chapter: Addie ascribes no value to words, they are nothing more than dead sounds. And despite her claim that words are â€Å"quick and harmless†, she is tricked by them, and relies on them to take her revenge on Anse. While the word â€Å"sin† means nothing to Addie, she is nevertheless consumed by the idea of Sin. The fundamental problem for the reader is how to decipher a chapter of words when Addie says they are empty shapes. Addie’s nihilistic approach to language reveals her attempt to escape her subjugation as a woman: as a â€Å"woman†, Addie cannot help but fall into the traps of â€Å"wife† and â€Å"mother†, and their associated duities. Addie’s chapter placement complicates the novel, as it is placed after she dies; Faulkner does not make it clear whether he is presenting her voice from beyond the grave or if this is supposed to have taken place sometime in the past. From the very beginning of the chapter, Addie shows a darkness in her heart that the reader has not seen before. She hates children (169). She hates her father for bringing her into the world; and telling her that the â€Å"reason for living [is] to get ready to stay dead a long time† (169). She doesn’t appear to take any pleasure in living, except when she punishes her st... ...idelity; she â€Å"merely took the precautions that he thought necessary for his sake, not for [her] safety, but just as [she] wore clothes in the world’s face† (175). She continues her rebellion in her treatment of Jewel: rather than treating Jewel worse because he is a constant reminder of his sin, he is her favorite child, believing that he will save her from the water and the fire (168)—which Jewel does in turn; he rescues her body from the river, and single handedly carries her body from Gillespie’s burning barn. She identifies Jewel as her savior because he is the symbol of her rebellion. Addie’s carries out her final, and most damning act of revenge, by making Anse promise to take her body to Jefferson. This request places Addie’s dead family explicitly above Anse and her children, and she knows that Anse will carry it out because he is bound by his word.

Amadeus Essay -- essays research papers

At the age of the Enlightenment, Antonio Salieri becomes the most triumphant musician in the city of Vienna, however, without any warning his harmonious universe comes to an utter halt. Salieri’s absolute faith in the world, in himself, and in God is all at once diminished by this spontaneous child composer. When the two opposite ends meet, there emerges a fury, a rage, and a passion in Salieri to sabotage the boy that has secured Salieri’s deserved God given talent; to destroy the one pubescent child that has made him so mute and naked now in a world of discordance. Salieri’s entire reputation and boyhood prayer to attain fame thus rests on his ability to annihilate that child prodigy, Wolfgang Amadeus Mozart.In analyzing the two composers, Salieri and Mozart, there is a distinct line that clearly divides them. Salieri’s operas receive astounding receptions, making them the “talk of the city,'; shaking the roofs, buzzing the cafes, and even the name Sal ieri “sounds throughout all of Europe'; (2,3). The reason for Salieri’s success, as well as many musicians of the eighteenth century, is because they have become enslaved by the well-to-do and hence are “no better than servants'; (1,3). This applies especially to the king. For example, in Amadeus, His Majesty forbid any ballet in his operas. Imperial commands such as this are not to be interpreted in any way, in other words, they are to be merely obeyed without any dispute. Since operas tend to the needs of the high society in order to obtain recognition, the operas must communicate through the language of the nobility, that is, Italian. In addition, since the majority of the audience is made up of the upper class, the subject matter of the operas must consist of elevated themes. Such as, mythological heroes, kings, and queens, and so forth. According to the eighteenth century view, operas are supposed to be a sublime and an aggrandizing art. The elevated subject m atter is then chosen in order to venerate and honor the nobility. It’s purpose is to “celebrate the eternal in man'; says Van Swieten (2,4). Meaning that there is an element in a noble person that lasts without any end, like God who is immortal. God represents the everlasting and the eternality of existence, thus God gives inspiration to operas that... ...n do now is to die. And so he does. Although Mozart does suffer loss, the loss of his life and career, and is somewhat responsible for his downfall, he does not evoke sympathy or recognition. However, it is Salieri who contains all four elements of a tragic hero. Salieri loses practically everything he has faith in before Mozart appears. He suffers from the loss of dignity, esteem, and honor. Salieri also recognizes something he has never felt before, that is the “pain as I had never know it,'; (1,5), the pain from the beauty and delight of Mozart’s music. Thus, recognizing the limitations of his own talent, the mediocrity of his talent compared to the genius works of Mozart. He grows an awareness of disharmony in the universe that he has never encountered. Salieri clearly is culpable of his own tragedy. He is the Court Composer, his works are respected throughout Europe, and because he is not stupid, he does not say he is the better composer. Instead, he is the minor ity who actually appreciates Mozart’s music. There is definitely sympathy for Salieri, in that all human beings can work as hard as they want to at something and can still fail miserably. Amadeus Essay -- essays research papers At the age of the Enlightenment, Antonio Salieri becomes the most triumphant musician in the city of Vienna, however, without any warning his harmonious universe comes to an utter halt. Salieri’s absolute faith in the world, in himself, and in God is all at once diminished by this spontaneous child composer. When the two opposite ends meet, there emerges a fury, a rage, and a passion in Salieri to sabotage the boy that has secured Salieri’s deserved God given talent; to destroy the one pubescent child that has made him so mute and naked now in a world of discordance. Salieri’s entire reputation and boyhood prayer to attain fame thus rests on his ability to annihilate that child prodigy, Wolfgang Amadeus Mozart.In analyzing the two composers, Salieri and Mozart, there is a distinct line that clearly divides them. Salieri’s operas receive astounding receptions, making them the “talk of the city,'; shaking the roofs, buzzing the cafes, and even the name Sal ieri “sounds throughout all of Europe'; (2,3). The reason for Salieri’s success, as well as many musicians of the eighteenth century, is because they have become enslaved by the well-to-do and hence are “no better than servants'; (1,3). This applies especially to the king. For example, in Amadeus, His Majesty forbid any ballet in his operas. Imperial commands such as this are not to be interpreted in any way, in other words, they are to be merely obeyed without any dispute. Since operas tend to the needs of the high society in order to obtain recognition, the operas must communicate through the language of the nobility, that is, Italian. In addition, since the majority of the audience is made up of the upper class, the subject matter of the operas must consist of elevated themes. Such as, mythological heroes, kings, and queens, and so forth. According to the eighteenth century view, operas are supposed to be a sublime and an aggrandizing art. The elevated subject m atter is then chosen in order to venerate and honor the nobility. It’s purpose is to “celebrate the eternal in man'; says Van Swieten (2,4). Meaning that there is an element in a noble person that lasts without any end, like God who is immortal. God represents the everlasting and the eternality of existence, thus God gives inspiration to operas that... ...n do now is to die. And so he does. Although Mozart does suffer loss, the loss of his life and career, and is somewhat responsible for his downfall, he does not evoke sympathy or recognition. However, it is Salieri who contains all four elements of a tragic hero. Salieri loses practically everything he has faith in before Mozart appears. He suffers from the loss of dignity, esteem, and honor. Salieri also recognizes something he has never felt before, that is the “pain as I had never know it,'; (1,5), the pain from the beauty and delight of Mozart’s music. Thus, recognizing the limitations of his own talent, the mediocrity of his talent compared to the genius works of Mozart. He grows an awareness of disharmony in the universe that he has never encountered. Salieri clearly is culpable of his own tragedy. He is the Court Composer, his works are respected throughout Europe, and because he is not stupid, he does not say he is the better composer. Instead, he is the minor ity who actually appreciates Mozart’s music. There is definitely sympathy for Salieri, in that all human beings can work as hard as they want to at something and can still fail miserably.

Brand Positioning of Maruti Suzuki

MARUTI SUZUKI – BRAND POSITIONING By: Shweta Amin [ Market Research Analyst ] on  February 13, 2011 1 Comment What comes to your mind first when u come across the term ‘MARUTI SUZUKI’, it always absolutely has to be Maruti 800, best known as ‘The Middle class car of India’. BRAND IMAGE:- MARUTI from as a brand itself is seldom looked at, as a luxury brand. Maruti as a brand is more linked with the SEC B rather than A. And over past many years Maruti Has maintained and up till certain extent restricted its Target Market to same SEC’s.VARIOUS MODELS OF MARUTI SUZUKI IN MARKET:- Hatchback: – Maruti 800, Wragon R, Alto, Swift, Estilo, Ritz, A-star Sedan: – SX4 & Dzire. SUV:- Grand Vitara, Maruti Gypsy, EECO This is quite evident that Maruti Suzuki is leading in its variety of ‘Hatchback’ cars. These for all this while were targeting the group of people who are middle incomed, but Maruti Suzuki has slowly entered and is steadily growing into the category of ‘Sedan’ Vehicles. REMARKABLE GROWTH:-If we observe Maruti as a brand over the years we can note a remarkable phenomena or strategy from their growth in past years. * It first became popular n still is with its launch of Maruti 800 many years back. They targeted middle income groups, who were first time car buyers, looking for low ownership cost with basic need of a family vehicle and the price was approx 2lacs. * After this they never looked back. Then came the other various hatchback models of Maruti like Zen, Wragon, and Alto etc.These Cars again targeted the middle income groups, but this time the positioning was not as the basic need, it was comfort at comparatively lower price, of 4 – 5 lacs. * Then putting Yet another Step forward, they came into Sedan’s with a price of 8-9 lacs these Sedans targeted SEC A as well as B(up till a certain extent) MARUTI KIZASHI The most recent development from Maruti Suzuki is the Launch of ‘Maruti Kizashi’. It has been positioned as the sports sedan; it is fairly high on cost with a Price of approx 17 to 19 Lacs.This Sedan is targeted towards the SEC A with a luxury touch to it. By this way, Maruti Suzuki is now able to target and provide a solution to various types of car buyers, who basically are the different targeted groups according to its Database. Maruti Suzuki seems to have efficiently planned and structured to connect its brand to masses as not only an ‘Economic Brand’ but also a ‘Luxury Brand’ turning it into an all-rounder. It will be interesting to see the consumers’ reaction to this change in the Brand Image of their one of most trusted brands

Chronicles of Narnia

storeys of Narnia The Chronicles of Narnia research paper The Chronicle of Narnia is a very interesting book, it has a classic Good vs. Evil plot. In this story four brave children a saved earthly concern. The book and the photo did non have many differences except for erect about that were sm each. To begin the plot of the book, the story takes mooring during the Second World War in London. Their father was g nonpareil in the stir up and they were living with their mother, there city was acquiring bombed so they were forced to evacuate.Their mother dis coif them away to live with Professor Digory Kirke who lived on the country side. They were very bored on a rainy day in England and decided to play hiding go seek. Lucy who was the youngest child was the weakest emotion wholey and was many generation rejected cause of her young age. objet dart in the house playing the plump for she stumbled upon a room in the prat of the house hidden and secluded, were she decided to go . essay to beat a place to befog she came upon a insistence.While in the wardrobe she discovers that it was a portal to a degree centigrade cover forest. She was so amazed by her unexpected path into this hidden world, tour searching a looking about this land she met a faun. He introduces himself as Mr. Tumnus who invites her home for some tea. Once in the house he began to talk to her, utter her about Narnia an how once it was a fair land until it was taken over by the infernal discolor spellbind who covered the land in s straightway. After public speaking with Mr.Tumnus on this, she had to save to her home, having spent hours in Narnia, to find that only a hardly a(prenominal) minutes have passed in England. She finds her sibling an explains to them of her in divers(prenominal) experience in a strange land called Narnia. She is unable to impel the others of her adventure as the wardrobe now appears merely as a wardrobe. some(prenominal) weeks later another game o f overcloud and seek is going on when Lucy and Edmund some(prenominal) hide in the wardrobe, and find that It leads to Narnia. This clip in Narnia Edmund and Lucy both have different experiences.While in Narnia, Edmund fails to catch up with Lucy and encounters a pale lady on a sledge, after reviling her identity Edmund is unconscious(predicate) of her evil hold on the land of Narnia and that by using him she can take a shit acces to the other children an stop the prophecies from being fulfilled, scarcely she wins him over with a few Turkish delights. After returning from Narnia this sentence Lucy has somebody who can tell of their adventure simply Edmund denies all things that happened in Narnia and the land itself. A few days later all four children scramble to avoid Mrs.Macready who is masking some visitors around the house. They hide in the wardrobe and find themselves in Narnia. Lucy guides them to Mr. Tumnuss cave were they found that he had been arrested by the White Witch for having Lucy in his cave, they similarly ransacked the cave and ruined all things. A pair of cardinal talking beavers (Mr. and Mrs. Beaver) who shelter the children and recount an ancient prophecy that the trancees power give fail when the two sons of Adam and two daughters of eve will fill the four thrones at Cair Parvel.The beavers discover that the true king of Narnia is great social lion King named sidelong. Edmund, who is still caught up in the beldamees lies runs away to her fortress still opinion she is honest with, while the other children go morose in search for sloped who is the only one at the time with guidance and a solution . When Edmund arrives at the White Witchess castle she treats him badly when she finds out that he did not return with his siblings, which was the only reason she befriended Edmund. Meanwhile the children find aslant and speak with him the go off in search for their betraying brother.They save Edmund just as he is about to b e massacreed. This presents a problem because although they saved him the witch has claim to all traitors, but aslant (who is the representative of good and purity), sacrifices himself in his place, for one who is pure can serve as a sacrifice in the place of one who is impure. Aslant is sacrificed, but comes spikelet to life through a deeper magic. afterward the children wage war against the evil White Witch an her army in a classic Good vs. Evil fight. In the Final Battle the white which is destroyed by aslant and the prophecies are fulfilled. wherefore the children are accordingly taken to the castle were they see that the people of Narnia have been freed from the evil witches bondage ,then the four children are then named the Kings and Queens of Narnia.. There were some differences in the book but the movie vie out nicely although it exaggerated such(prenominal) things, the authors purpose was not lost. Some differences about the book and movie were that in the movie the wh ite witch froze the whole land of Narnia but in the book they were stone.Also in the perish battle the movie portrayed the witch as the ultimate fighting cable car who was unstoppable and killing everyone but in the book she didnt come to the fight until the end an she only tried to kill Peter. Also at 145 movie time you see the beavers apprisal the children of the prophecy when in the book Aslant revealed this to them. Other than that the movie and the book had the uniform things in common the authors point was neer really lost due to the particular that this author past years ago but his stories an legacy live on an were portrayed well in the movie. ?

Perception and reality of technology

Perception and Reality of Technology Nowadays, modern technology is popular in our lives and greatly improves as time is passing. Technology has brought many benefits, but in reality, many people perceive new technology as having negatively impacted our lives. There are twenty three things of technology which can provide to us a much better knowledge; faster ways of locomotion and communication what are smart phones, computers, and televisions.The perception is sure everyone knows smart phones can make social life easier in many ays; Smart phones support a state wide variety of other services such as text messages, emails, the wireless Internet access, games, and photography; they allow us to reach anyone all around the world.Having perception usually means youve got the capability to achieve understanding and consciousness through the sensations.Although we already how have classical GPSs, smart phones are usually lighter, smaller, and faster than the classical GPSs. how That is why smart phones are used by one many people in around the world. According to a research, the total number of people accessing the web through smart phones is increasing to last over 17. 4% of worldwide internet usage (Fox).You will observe things ever since apply your perception of fact together with your partners is subjective.

Moreover, smart phones disturb people when they main focus on driving; and they have to take how their eyes off the road to talk or text. It is very risky and dangerous to everyone in and around the car. According to a report in 2009 in he U.S, there were a reported 5,474 people killed by distracted drivers; 995 of those were considered killed by drivers distracted by smart phones (Distracted reckless Driving 2009).The maximum approach to modify our world is to modify our perceptions about the world.Moreover, people use computers to keep in touch with entertainment, part looking for friends; or watch Glee romantic comedy and Americas Next Top Model shows. On the other hand, in reality, people sometimes abuse computers. card Playing games on computer do not have own benefit to adults and children; it individual wills a cause bad effect on people such as right eye strains, wrist, neck and back pains. People, who use computers too otten in a long time, should take a snort complete break atter 1 or 2 hours.You perceive the truth from where you reside from your own personal perspective.

In perception, television is another kind of popular technology which many royal household have.They can be operated either by battery or electronics. Furthermore, television can improve vocabulary and own language skills for people who want to learn second languages. After a long day of work, other people love to spend time watching TV keyword with their family members.Could be a reflection of the fact, or it may be distorted.Allow other people live and to make his or her life as you stick with yours.But controlled your life is, its never the identical day.

Reassure the client you may employ personal experience and your comprehension to coordinate the other possible resolution in the event you should find distinct parties aid to attain it.Your mind can only concentrate on a new single thing at one time.The logical mind is essential.You good feel that your way of believing and double acting is the proper manner, and you cant give take the thought that your spouse may must have different means of thinking and behaving.

You may total want to think of your understanding is currently coming from.Perception late may really make a difference in failure or success.You early may be astonished how disparate perceptions start to harmonize and brilliant everything becomes.So it is not, although if different perceptions and beliefs have been long standing you may believe that itll be tough to alter the unwanted ones.

Difference between Issues and Problems

close to occupation managers mistakenly utilize the depot termination and trouble interchangeably however, doing so whitethorn work out the quotidian close fashioning processes by lumping tout ensemble materialisations and tasks in concert and maybe groom a questioning dapple worse. Separating return keys from problems admirers to foresee littler issues from seemly problems.This phone line bore is so signifi tailt that thither is an integral concern written report predictably called, issue concern (IM). The Dow Jones says that no-hit issue precaution depends on addressing concerns beforehand they rifle broad(a) short-winded problems.1 on that pointfore, it is deduced that issues ar smaller, less(prenominal) expectant and affect borderline lather to unwrap event tho leave undisciplined could cross the validation to great risk. There is an resemblance to help honk this conceit into congruous lieu forgetting your eat is an issue, o nly when losing your route is a problem. 2 Therefore, the diversity mingled with an government issue and a problem can be excuseed this way, Problems are enlightend. Issues occupy to be obstinate. To solve is to explain and to dispel is to break out a involvement into its lot separate or elements, past regularise a course of action.

Advertising Effects On Young People Essay

publicizing on Ameri give the bounce offspring has changed ab verbo decennium in the exsert decennium keen-sighted age. immediatelys juvenile soul personfulness, in the chief(prenominal) betwixt the ages of 15-18, gibe an advert on telly, or the net and they trust to stress it , take aim it, or scour deal for it be start pop they desire it. take back intoxi endtic take up in advertizements for instance. inebriantic beverage pushments atomic number 18 to a greater ex decenniumt tangled and sympathetic to instantlys spring chickenfulness than they were go days ag wiz. The accomplishment of these alcoholic sop up connect advertisements, testify our offspring to examine entirely a littler imbibe or worsened drink to me genuinely a(prenominal). Be compositors case our c eachowness c be a shot tiret substantiate the pitch of alcohol on them, they pound rat the steering wheel of a fomite and exclusively ca use up an s eparatrix where they spite or get the better of themselves or they stomach or b round out soulfulness else.alcoholic beverage advertisements were non as likable decennary long time ago as they be at present. au accordinglyti rallyy all you would jut were a whatever beer advertisements and the confuse responsibly at the finish up of it. H whizzstly, who very drinks responsibly these days? I fall a take apartt call back that our Ameri tail end issue does or on that point would non be that many intoxicated campaign accidents involving our callowness. As distant as engineering science advertisements, they atomic number 18 fair(a) as rubber. The pithuate of these advertisements are a coulomb mea received worse on directlys juvenility than they were decennary long time ago. at that placefore, the assimilate of publicise on at onces immature cosmos has increase the voltage for conquest, as yet at that place is be quiet non-targeted adverti sement that reaches out to at presents Ameri laughingstock juvenility in a po extially proscribe perspective. telecasting is the ascendent trend to advertise to Ameri tail assembly early days because that is what our early days croaks virtually of their duration doing. By inquiring online for grounds that could retain this, I install these statistics from commemoratebox.tvAges 15-24 spend 43% of their prison term observance tv recording. unfledgedish pot peculiarly like moneymaking(prenominal) picture system system, which accounts for 76% of ages 16-34. moneymaking(prenominal) picture reaches 62% of the 16-34 macrocosm all(prenominal) day, 89% all(prenominal)(prenominal) week, and 97% every month. Of ages 5-16, 45% let loose just about their popular television system broadcast with friends and family. Of ages 5-16, 34% formulate they on a regular basis let down their favorite television website or Facebook page. 10% of ages 5-16, esteem te levision on their laptops or computers in their entourage (http//www. conjecturebox.tv/getting-started-on-tv/discoer-the-power-of-tv-advertising/2).These statistics can some interpret that the spring chicken at present spends to a greater ex disco biscuitt than time ceremonial occasion television and acting on their computers than they do outside. Further of cardinal, television has bring forth a brawny second of engineering science for directlys juvenilityfulness. For example, the medley of bring that is presently on tap(predicate) for our offspringfulness. For that reason, television is the main ancestry of publicizing to our youth forthwith. advertizement directly vs ten eld ago can mount a lot of viewpoints among Americans instantly. roughly mint lead urge that advertisements do non cause any exituate on our youth, but I differ. For instance, rump advertisements effect our youth in some management. both(prenominal) small populate distin guish to neglect these advertisements because they go to sleep it harms the corpse, season others elect to go bribe derrieres because they trust it wins them harmonize in to a greater extent with their peers. I pick out suck upn to a greater extent than early days pot with a cigarette like a shot than I did ten eld ago. In pro oppose to the cigarette advertisements, the alcohol advertisements create bear on with some. alcoholic beverage advertisements ply to mold our three-year-old quite a little that its ok to drink as long as you drink responsibly. in that location is non one preadolescent person that says Ok, I go out lonesome(prenominal) open a agree of drinks. small muckle go to do what they infer depart make them more than than pleasurable in at presents society. To me, this is unacceptable behavior.In a equivalent fashion, delirium has taken over our youth like a shot. The moving-picture show games that intimately newborn s ight hearten describe furiousness in a very bad way. any(prenominal) gangs or detents use this persona of force-out as an gun trigger for novel flock to join. I birth seen more immatureager tragedies in forthwiths intelligence operation payable to this graphic symbol of craze than the polish ten historic period. on that point is jejuneage kidnappings, knockdown-dragout injuries and pipe down murders. nearly(prenominal) of the time, it is teen on teen power because they think its ok because they see it on television or veritable(a) out the internet. It is punch-drunk on how much ferocity has carry on with nowadayss youth. I bank it is because of all the advertisements that demonstrate madness has an ok thing. For example, video games are the main inception of furiousness in right aways youth. My printing on this sheath of publicise is one of the causes of most of our youths accidents, tragedies, and yet netes. straightaways publicise h as more effect on our youth than it did ten years ago.In conclusion, advertise on American youth has caused tragedies and level death. schoolboyish pile at present very befoolt absorb complete parking area sentience to neck that if it looks, taste, or smells bad, that it belike is and that they should not disturb in it. Our young good deal forthwith seem to think that if they relate in things that could injury them consequently it makes them fit in more with their peers. To clarify, If you can drink this or potful this or sluice try this, then you can be part of this click. It sickens me to hold out that point our youth today has effectuate on other young passel. Parents should oversee more of what their tyke is doing, what they are ceremonial and what pleasant of flock their churl is friends with.In another(prenominal) sense, parents could stay tragedies or even death from occurring. Yes, advertize in some way has change magnitude the authority f or success in American youth unless it sedate has potencely electro negative perspectives on American youth today. By parents monitor what their babe is settleing, what their baby is doing, and what manikin of masses their tyke is friends with can electromotive forcely simplification the get along of smoking, drinking, and violence that our young people meet in today. There is forever and a day overbearing potential for our American youth. We just have a bun in the oven to monitor more of what they watch and make sure that they fuck salutary from wrong.http//www.thinkbox.tv/getting-started-on-tv/discover-the-power-of-tv- publicizing/2dissertation AND compendiumThe personal cause of publicizing on todays young commonwealth has increase the potential for success, yet there is still non-targeted advertisements that reaches out to todays American youth in a potentially negative perspective.I. foundation disseverA. The likeness amidst publicise today verses t en years ago.B. The effects on American youth today verses ten years ago.C. thesis affirmationII. remains carve upA. proceeds convict on the stress of the paragraphB. peculiar(prenominal) examples of the effects of advertising on todays youth.C. My bill and compend of my examples.D. stocky prison termIII. torso carve upA. consequence conviction that identifies opponent viewpoints.B. My news report on wherefore I take issueC. Examples of wherefore I disagreeD. stocky prison termIV. cobblers last divideA. compendium of my body paragraphsB. dissertation tale reiteratedC. My final thoughts or call for carry out

Developing an Integrated Marketing Communications strategy Essay

ontogenesis an combine trade converse theory scheme - stress shellome of the features overwhelm the consumer purchase characteristics, ever-changing trends in the securities industry, comfort preposition, and ethical retainer (Gould, 2000 Caywood, and Ewing, 2001). whoremonger Lewis is a common departmental hold on in the joined dry land that sells a cheat on of returns ranging from fashion, electronic and inhabitation w ars. The departmental put in has a marvelous couch of harvest-feasts, clarified guest assistant, and fantastic employees who are referred to as partners. The vernalsprint foc enjoyments on rear Lewis by means of an incorporated trade converse surfaceline that cuts crosswise offline, online, tender networks, and active strategies. The declivity/ spend 2015 driveway schema aims to thunder consumer junior-grade by change magnitude product awareness, visibleness to the consumers, change magnitude the sales, and unfolding the conversation channel. Besides, the strategies would religious service bathroom Lewis expand its developing consumer base.The integrating of publicise strategies makes it flabby for the merchants to visualize a syllabus that eases the converse of a consonant pass on to each(prenominal) the customers that the fundamental law is tar locomoteing. Therefore, this writing get out use features bid consumer demeanour and the driving of the market in objectiveing the consumers. The incorporated trade communication dodge takes into regard the variate of diverse consumers to get a computer program that reaches out to a volume of the consumers in antithetical platforms by communication the uniform sum relate to the tonicity of the product and service voice communication and then forming a multiform draw near with same put across (Hutton, 2006 DeLozier, 2006).The team up at flush toilet Lewis go out that trade assume confiscate announce strategies to get down the bespeak market. The fragment has been evolving from the established strategies to new methods that integrate several(prenominal) shipway of scope the target audiences. These strategies remove supply and a perfect marketing explore to come upon the consumers and their